@article { author = {Karim-Nezhad, Ghasem and Khorablou, Zeynab and Sadegh, Bayat and Mahmoudi, Tohid}, title = {Electro-Polymerized Poly(L-Arginine) Film as An Efficient Electrode Modifier for Highly Sensitive Determination of Methadone in Real Samples}, journal = {Analytical and Bioanalytical Electrochemistry}, volume = {14}, number = {8}, pages = {730-741}, year = {2022}, publisher = {Analytical and Bioanalytical Electrochemistry is an international scientific journal, which is published online every 3 months (since 2009), every 2 months (since 2011) and monthly (since 2018) by Center of Excellence in Electrochemistry, University of Tehran}, issn = {-}, eissn = {2008-4226}, doi = {}, abstract = {Developing simple yet efficient sensors for drug-abuse detection is highly required from both human health and medical aspects. In this work, a glassy carbon electrode modified by a thin layer of poly L-arginine (P-L-Arg/GCE) was fabricated by electropolymerization of L-arginine monomer using cyclic voltammetry (CV) and then it was used for determination of Methadone (MET). The surface characteristics of the P-L-Arg/GCE was examined by scanning electron microscopy and electrochemical impedance spectroscopy. To study the electrochemical responses of MET on P-L-Arg/GCE and bare GCE, cyclic and differential pulse voltammetric techniques were employed. For P-L-Arg/GCE, a considerable enhancement in electrooxidation signal of MET was observed compared to bare electrode. The influence of various determinant parameters on P-L-Arg/GCE performance was studied. At optimized conditions, using DPV technique, the electrooxidation peak current was linear to MET concentration in the ranges of 0.49-2.98 µM and 2.98-11.9 µM with a detection limit of 0.32 µM. The utility of P-L-Arg/GCE was successfully validated by analyzing MET in serum and urine samples, representing its potential application for real samples analysis.}, keywords = {Methadone,Arginine,Electropolymerization,Modified glassy carbon electrode,Differential pulse voltammetry}, url = {https://www.abechem.com/article_254432.html}, eprint = {https://www.abechem.com/article_254432_75ad3d79d9fe1659fd519593063ce6f7.pdf} }