%0 Journal Article %T A New Efficient Agarose Gel based Electro-membrane To Extract, Preconcentrate, Clean-up and Quantify Nilotinib in Biological Samples %J Analytical and Bioanalytical Electrochemistry %I Analytical and Bioanalytical Electrochemistry is an international scientific journal, which is published online every 3 months (since 2009), every 2 months (since 2011) and monthly (since 2018) by Center of Excellence in Electrochemistry, University of Tehran %Z - %A Bameri, Amir Ehsan %A Zeraatkar Moghaddam, Ali %A Ghiamati, Ebrahim %D 2021 %\ 12/01/2021 %V 13 %N 3 %P 408-422 %! A New Efficient Agarose Gel based Electro-membrane To Extract, Preconcentrate, Clean-up and Quantify Nilotinib in Biological Samples %K Agarose gel %K Clean up %K Electro-membrane extraction %K Pre-concentration %K Nilotinib %R %X Abstract- For the first time, this study used agarose gel as a membrane in electro membrane extraction (AG-EME) without using any organic solvent to preconcentrate and clean up nilotinib in biological samples, following: its spectrofluorometric determination. Optimal conditions were: agarose concentration (w/v) 3.0%, acetic acid concentration in membrane (v/v) 0.2%, applied electric voltage 50V, pH of the donor phase (pHd) 4, pH of the acceptor phase (pHa) 3, and extraction time of 30 min. Limits of detection (LOD) and quantification (LOQ) were 15 and 50 ng mL˗1, and the analytical curve was linear at the range of 50–5000 ng mL˗1. The proposed validation method was compared with the other electro-membrane extraction techniques. Our proposed method is fast; it uses little sample and possesses a short extraction time. The developed procedure could be utilized successfully to determine the total amount of nilotinib, an anticancer drug, as a routine analysis in the biological samples. %U https://www.abechem.com/article_248525_b5c5dd680e2935df65cd0ffbd4fe1858.pdf