%0 Journal Article
%T Calibration Curve Approaches for Nonlinear Data Points Obtained in Colo 320 Exosomes Determination
%J Analytical and Bioanalytical Electrochemistry
%I Analytical and Bioanalytical Electrochemistry is an international scientific journal, which is published online every 3 months (since 2009), every 2 months (since 2011) and monthly (since 2018) by Center of Excellence in Electrochemistry, University of Tehran
%Z -
%A Akay Sazaklioglu, Sevda
%A Torul, Hilal
%A Kabadayi, Hilal
%A Vatansever, Hafize Seda
%A Tamer, Ugur
%A Celikkan, Huseyin
%D 2022
%\ 11/30/2022
%V 14
%N 11
%P 1027-1043
%! Calibration Curve Approaches for Nonlinear Data Points Obtained in Colo 320 Exosomes Determination
%K Nonlinear calibration curve
%K Exosome determination
%K electrochemical impedance spectroscopy
%K Mathematical models, Biosensors
%R
%X The limit of detection (LOD) is defined as the lowest quantity or concentration of a component that can be reliably distinguished from the limit of blank (LOB). LOD value is one of the most important parameters considered for many determination methods and is usually calculated on the linear correlation between signal and concentration. However, the linear correlation may not always be obtained in experimental studies. We claim that data with low linear correlation have meaning, and we present such a study because analytical studies based on these data are not easily understood in the literature. In this manuscript, we suggest that a calibration curve can be obtained from nonlinear data points and the LOD value can be calculated. We tested this approach for the determination of exosomes and supported it with mathematical calculations. We produced a label-free sensor using anti-CD63 on the gold electrode for selective and reliable impedimetric detection of the exosomes obtained from Colo 320 cell lines in data points that are high concentrations and out of linearity. We characterized in detail what each calculation means. This sensor with a LOD value of 3.90×1011 exosome particles µL-1 and with a cubic polynomial model for the calibration curve was considered sensitive and reliable, especially for high vesicle content of samples such as cell culture medium.
%U http://www.abechem.com/article_698015_f4fe9fac504f669c7615ca3830bd1eb0.pdf