Voltammetric Determination of 5-Hydroxyindoleacetic Acid at Poly (p-amino benzene sulfonic acid) Modified Sensor

Document Type : Original Article

Authors

1 İnönü University, Faculty of Science, Department of Chemistry, 44280 Malatya, Turkey

2 İnönü University, Faculty of Pharmacy, Department of Basic Pharmaceutical Sciences, Division of Analytical Chemistry, 44280 Malatya, Turkey

Abstract

In this study, the quantitative amount of 5-hydroxyindoleacetic acid (5-HIAA) was determined at poly (p-amino benzene sulfonic acid) (p-ABSA) modified glassy carbon electrode. The redox property of 5-HIAA was investigated by cyclic voltammetry. The cyclic voltammograms showed that the redox reaction of 5-HIAA was irreversible. Scan rate study showed that the redox reaction of 5-HIAA was controlled by both diffusion and adsorption on the poly (p-ABSA) modified sensor. Differential pulse voltammetry technique was used for the quantitative analysis of 5-HIAA in phosphate buffer solution at pH 7.00. The linear working range of the calibration graphs was determined as 1×10-5–9×10-5 M (R2, 0.9912), and the detection limit was determined as 5.3×10-7 M. Recovery values ​​in the analysis of urine samples were between 99.4% and 103.0%. The results showed that the modified sensor can be applied to the determination of 5-HIAA in the presence of ascorbic acid. The proposed sensor is promising for routine analysis due to its high selectivity, reproducibility, long-term stability, and high recovery values in biological samples.

Keywords

Analytical and Bioanalytical Electrochemistry
Volume 14, Issue 4
April 2022
Pages 418-431

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History

  • Receive Date: 18 January 2022
  • Revise Date: 07 March 2022
  • Accept Date: 08 March 2022

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