Document Type : Original Article
Department of Chemistry, Dehloran Branch, Islamic Azad University, Dehloran, Iran
Medical Biology Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
Nutrition Health Research center, Hamadan University of Medical Sciences, Hamadan, Iran
In this paper, we developed a simple and disposable electrochemical paper-based immunosensor (e-PI) based on manual screen printing method via patterned sticker label film on paper. The proposed electrochemical sensors provide the opportunity for economical single-use analysis of biological samples. The e-PI was constructed with hydrophobic glossy paper layers on a wax paper substrate to define three-electrode system. A carbon ink was used for construction of e-PI which composed of the graphite powder and cellulose acetate. The stability of the e-PI was evaluated using electrochemical methods. Carbohydrate antigen 15-3 (CA15-3) antibodies to the target CA15-3 antigen were immobilized on gold-modified graphite electrodes on the e-PI. Electrochemical properties of e-PI were investigated by cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. The fabricated e-PI offers a vast linear range from 0.5 to 200 U mL−1 with a low limit of detection 0.15 U mL−1. Furthermore, the fabricated e-PI has acceptable stability, accuracy and high sensitivity.