@article { author = {Ghafouri, Vahid and Banaei, Aghdas}, title = {A Novel Choline Biosensor Based on Immobilization of Enzyme Choline Oxidase on the β-Ga2O3 Nanowires Modified Working Electrode}, journal = {Analytical and Bioanalytical Electrochemistry}, volume = {12}, number = {5}, pages = {663-687}, year = {2020}, publisher = {Analytical and Bioanalytical Electrochemistry is an international scientific journal, which is published online every 3 months (since 2009), every 2 months (since 2011) and monthly (since 2018) by Center of Excellence in Electrochemistry, University of Tehran}, issn = {-}, eissn = {2008-4226}, doi = {}, abstract = {This article is a report on a novel and high-stability biosensor with minor interference effects of surface modification of working electrode with β-Ga2O3 nanowires (NWs) on choline oxidase biosensor were investigated in an electrochemical detection system. β-Ga2O3 NWs were materialized on the silicon substrate in a catalyst-free growth mechanism. The β-Ga2O3 NWs were in string form with 10 μm in length and uniformly 30 nm in diameter. Then the enzyme choline oxidase (ChOx) immobilized on the β-Ga2O3 NW/CB arrays on the working electrode. The Cyclic voltammetry (CV), Impedance spectroscopy and differential pulse voltammetry (DPV) measurements were performed for bio-sensing detection with choline chloride as substrate. One of the most prominent features of this surface modification with Ga2O3 NWs /CB is that the peak intensity, which is often of the order of μA, is greatly increased and reaches about mA. Chronoamperometry amplification well confirms the performance of this surface modification. The current with the CB modified electrode is about three times more than the non-modified electrode, and Ga2O3 NWs/CB modified electrode twice more than the CB modified electrode. The maximum current in the DPV data is modelled linearity iap= 0.159 (C)+2.28 and R2 = 0.982. The limit of detection (LOD) of the electrode for the choline measurement was reached to 8.29 μM. The Sensitivity of the electrode was around 0.0397 mA mM−1 mm−2. The stability of this biosensor has been well studied over a period of 6 months, and more than 80% of the permanency of its response has been confirmed.}, keywords = {Ga2O3 Nanowires,Electrochemical biosensor,Choline oxidase,Screen-printed electrode,Amperometry}, url = {https://www.abechem.com/article_39954.html}, eprint = {https://www.abechem.com/article_39954_71fec68ee1eac1e78c76fef269c64310.pdf} }