Analytical and Bioanalytical Electrochemistry is an international scientific journal, which is published online every 3 months (since 2009), every 2 months (since 2011) and monthly (since 2018) by Center of Excellence in Electrochemistry, University of Tehran Analytical and Bioanalytical Electrochemistry - 17 2 2025 02 28 Modified Carbon Paste Electrode Using MIL-101 (Fe) Metal-Organic Framework and Ionic Liquid for Ferrocene-Mediated Voltammetric Determination of Glutathione 190 203 721576 10.22034/abec.2025.721576 EN Ali Rezaeizadeh Department of Biological Science, Sirjan Branch, Islamic Azad University, Sirjan, Iran Afsaneh Hajializadeh Department of Natural Resources, Sirjan Branch, Islamic Azad University, Sirjan, Iran 0000-0001-5688-1511 Journal Article 2024 10 11 This study presents a novel electrochemical sensing technique for the quantitative analysis of glutathione levels, a crucial biomolecule linked to various medical conditions such as diabetes, Parkinson's disease, and cancer. The increasing demand for simple, rapid, and cost-effective assays in clinical diagnostics drives the need for innovative solutions. We synthesized the metal-organic framework (MOF) MIL-101(Fe) via a solvothermal method and characterized using Fourier-transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM), and energy dispersive x-ray spectroscopy (EDX). Then, a modified carbon paste electrode using MIL-101 (Fe) MOF and ionic liquid (IL) for ferrocene (FC)-mediated electrochemical determination of glutathione was prepared (MIL-101 (Fe) MOF/FC/ILCPE). The electrochemical performance of the designed electrode was assessed through cyclic voltammetry (CV), linear sweep voltammetry (LSV), chronoamperometry, and differential pulse voltammetry (DPV). Based on the CV investigations, the as-prepared MIL-101 (Fe) MOF/FC/ILCPE demonstrated good electrocatalytic performance towards the oxidation of glutathione. The developed sensor demonstrated a broad linear dynamic range of 0.5–385.0 µM for glutathione detection. The limit of detection (LOD) was calculated as 0.15 µM. Practical applications were validated through the analysis of human urine and hemolyzed erythrocyte samples, showing exceptional effectiveness in glutathione detection. This electrochemical sensor exhibits significant potential for clinical applications in monitoring glutathione levels. https://www.abechem.com/article_721576_50616fce5b3defc090889ccf499ce9c2.pdf