Selective Electrochemical Nanosensor based on Modified Carbon Paste Electrode for Determination of NADH in the presence of Uric Acid

Authors

Department of Chemistry, Faculty of Science, Yazd University, Yazd, 8915818411, Iran

Abstract

The electrochemical properties of a modified carbon paste electrode with the synthesized compound of 2,2'-[1,7–heptanediylbis(nitrilomethylidene)]-bis(4-hydroxyphenol) (DHBH) and graphite nanoparticle (GN) were studied by cyclic voltammetry (CV), chronoamperometry and differential pulse voltammetry (DPV) methods. The proposed electrode shows excellent electrocatalytic activity towards the oxidation of NADH under the optimum pH of 7.0. The modifier and nanoparticle simultaneously lead to a reduce overpotential of NADH oxidation about 250 mV and enhance current about 6 μA of the unmodified CPE. This electrochemical sensor exhibited a detection limit (3σ) of 13.4 nM with two linear dynamic ranges (0.01-6.0 and 6.0-400.0 μM) for the determination of NADH. The modified electrode can detect well NADH (180 mV) in the presence of Uric acid (360 mV) simultaneously. Also, the performance of the proposed sensor was evaluated in real samples.

Keywords

References

[1] Z. Taleat, A. Khoshroo, and M. Mazloum-Ardakani, Microchim. Acta 181 (2014) 865.
[2] M. Mazloum-Ardakani, F. Sabaghian, A. Khoshroo, and H. Naeimi, Chinese J. Catalysis 35 (2014) 565.
[3] M. Mazloum-Ardakani, F. Sabaghian, A. Khoshroo, M. Abolhasani, and H. Naeimi, Ionics 21 (2015) 239
[4] N. Sattarahmady, H. Heli, and S. Moradi, Sens. Actuators B 177 (2013) 1098.
[5] S. Serban, and N. El Murr, Biosens. Bioelectron. 20 (2004) 161.
[6] A. Radoi, and D. Compagnone, Bioelectrochemistry 76 (2009) 126.
[7] I. Ali, T. Khan, and S. Omanovic, J. Mol. Catalysis A 387 (2014) 86.
[8] F. Ricci, A. Amine, D. Moscone, and G. Palleschi, Biosens. Bioelectron. 22 (2007) 854.
[9] D. P. Curran, T. L. Fevig, C. P. Jasperse, and M. J. Totleben, Syn. lett. 1992 (1992) 943.
[10] S. Liu, Z. Du, P. Li, and F. Li, Biosens. Bioelectron. 35 (2012) 443.
[11] H. Li, R. Li, R. M. Worden, and S. C. Barton, ACS Appl. Mater. Interf. 6 (2014) 6687.
[12] M. Mazloum-Ardakani, M. Zokaie and A. Khoshroo, Ionics 21 (2015) 1741.
[13] I. H. Fox, Metabolism 30 (1981) 616.
[14] D. Lakshmi, M. J. Whitcombe, F. Davis, P. S. Sharma, and B. B. Prasad, Electroanalysis 23 (2011) 305.
[15] C. R. Raj, F. Kitamura, and T. Ohsaka, Analyst 127 (2002) 1155.
[16] M. Mazloum-Ardakani, F. Sabbaghian, B.-F. Mirjalili, and M. A. Sheikh-Mohseni, Anal. Bioanal. Electrochem. 6 (2014) 106.
[17] E. Laviron, J. Electroanal. Chem. Interf. Electrochem. 52 (1974) 395.
[18] M. Sharp, M. Petersson, K. Edström, and J. Electroanal. Chem. Interf. Electrochem. 95 (1979) 123.
[19] A. J. Bard, and L. R. Faulkner, Electrochem. Methods 2 (2001) 482.
[20] E. Sharifi, A. Salimi, and E. Shams, Biosens. Bioelectron. 45 (2013) 260.
[21] M. Govindhan, M. Amiri, and A. Chen, Biosens. Bioelectron. 66 (2015) 474.
[22] S. Han, T. Du, H. Jiang, and X. Wang, Biosens. Bioelectron. 89 (2017) 422.
[23] S. Mutyala, and J. Mathiyarasu, J. Electroanal. Chem. 775 (2016) 329.
[24] M. Eguílaz, F. Gutierrez, J. M. González-Domínguez, M. T. Martínez, and G. Rivas, Biosens. Bioelectron. 86 (2016) 308.
Analytical and Bioanalytical Electrochemistry
Volume 12, Issue 2
February 2020
Pages 277-288

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History

  • Receive Date: 16 January 2020
  • Revise Date: 29 February 2020
  • Accept Date: 29 February 2020

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