Electroanalysis of Orphenadrine Hydrochloride by Graphene Modified Glassy Carbon Electrode and Its Oxidation Mechanism

Patil, Shreekant M; Bagogi, Atmanand M; Konnur, Santosh B; Gokavi, Naveen M; Nanadibewoor, Sharanappa T

Electroanalysis of Orphenadrine Hydrochloride by Graphene Modified Glassy Carbon Electrode and Its Oxidation Mechanism

Document Type : Original Article

Authors

Shreekant M Patil

Atmanand M Bagogi

Santosh B Konnur

Naveen M Gokavi

Sharanappa T Nanadibewoor

P.G. Department of Studies in Chemistry, Karnatak University, Dharwad-580003, India

Abstract

Electroanalysis of orphenadrine (ORD) by graphene modified glassy carbon electrode (GPN/GCE) was studied using cyclic voltammetric and linear sweep voltammetric (LSV) techniques. The variation of the current with pH, concentration and scan rate was investigated to optimize the experimental condition for determination of ORD. The optimal pH value for voltammetric determination of ORD is the physiological pH 7.0. The electrochemical behavior of the ORD at GPN/GCE was a diffusion-controlled process. Under the optimal conditions, the anodic peak current was linearly proportional to the concentration of ORD in the range from 1.0 × 10-7 to 1.2 × 10-6M with a limit of detection 2.8 nM for LSV. This method was applied for quantitative determination of ORD levels in urine as real samples. Further interference study was also carried with some common interfering substances. The present method could possibly be adopted for the pharmacokinetic studies as well as for quality control laboratories.

Keywords

Orphenadrine

Voltammetric

Graphene

Glassy carbon electrode

Oxidation