In the present investigation, a potentiometric sensor using molecularly imprinted polymer (MIP) of Cefotaxime sodium (CS) drug was produced. The MIP was synthesized using CS as a drug, Meta acrylic acid as a monomer, and triethylene glycol as a cross-linker. Furthermore, potentiometric sensors were produced by dispersing CS MIP in Ortho-nitro phenyl octal ether and reinforcing it into a PVC matrix (PME) and PVC coated on graphite (CGE). There was a linear electrode response ranging from 3.0×10^–7 to 1.0×10^–2 M having 57.9 mV decade^–1 as slope and a limit of detection of 9.0×10^–8M for CGE. The pH-independent region ranged from 4.5 to 9.2, and the flow injection analysis (FIA) system depicted a lifetime exceeding eight weeks. The separate solutions technique was utilized to determine the selectivity coefficients for numerous ions. As indicated by our results, the selectivity coefficients for the entire implemented anions, had a magnitude of ≤10^–3. The design and assembly of the flow cell were not complicated and extended usage did not overburden the memory. The suggested sensor was successfully used to directly ascertain the levels of both real and synthetic Cefotaxime samples.