Two-Step Protein-Film Voltammetry Associated with Intermediate Reversible Chemical Reaction-Diagnostic Criteria for Characterizing Systems with Inverted Potentials in Square-Wave Voltammetry

Document Type : Original Article


Faculty of Medical Sciences, Goce Delcev University Stip, Republic of Macedonia


Many electron transfer mechanisms of important physiological systems commonly occur as multistep electrode reactions that are initiated by a gain or loss of an electron. The product of initial electrochemical reaction might subsequently participate in chemical and/or electrochemical reaction(s), in which the final product of entire mechanism is generated. Therefore, a proper understanding of electron transfer phenomena is of outmost importance to get information about mechanism going on in the redox transformation of relevant physiological systems. In this work, we focus on theoretical voltammetric features of complex multielectron surface electrode mechanisms, in which two electron transfer steps are bridged by a reversible chemical reaction. Special attention is paid to systems with so-called “inverted potentials”, in which the second electron transfer requires less energy to occur than the first one. Square-wave voltammetry (SWV) of so-called “surface ECrevE mechanism” is explored as a valuable technique that can give relevant information about diagnostics of this mechanism. The presented model is suitable to study the activity of various enzymes and lipophilic organic compounds by exploring the “protein-film voltammetry” scenario.