An Electrochemical Strategy for the Highly Sensitive Voltammetric Determination of a Cardiovascular β-Blocking Agent in Biological Fluids

Megalamani, Manjunath; Patil, Yuvarajgouda; Abbar, Jyothi C; Nandibeewoor, Sharanappa

An Electrochemical Strategy for the Highly Sensitive Voltammetric Determination of a Cardiovascular β-Blocking Agent in Biological Fluids

Document Type : Original Article

Authors

Manjunath Megalamani ¹

Yuvarajgouda Patil ¹

Jyothi C Abbar ²

Sharanappa Nandibeewoor ¹

¹ Department of Chemistry, School of Advanced Sciences, KLE Technological University, Hubballi, Karnataka, India

² BMS Institute of Technology and Management, Bengaluru-560064, Affiliated to Visvesvaraya Technological University, Belagavi, Karnataka, India

Abstract

In this report, different voltammetric tools such as cyclic, linear sweep, and square wave voltammetry were used to investigate acebutolol, a -blocker type of drug that is used to treat a variety of ailments, including cardiovascular disease, using a carbon paste electrode (CPE) under physiological environment. Under various phosphate buffer solutions, the voltammetric behavior of acebutolol displays two oxidation peaks and one reduction peak potentially within the range of -0.2 to 1.4 V, optimum results were obtained for pH 7.0. The electrode reaction was accompanied by two protons and two electrons transferred respectively; a probable electro-oxidation pathway has been proposed. The square wave voltammetric technique (SWV) was applied to perform a quantitative analysis of acebutolol. The linearity range was found to be between 0.06-10 µM. The LOD and LOQ were 2.06 nM and 6.88 nM, respectively which were superior to earlier methods. Furthermore, the new approach was used to determine the amounts of acebutolol in biological samples.

Keywords

Drug analysis

Carbon paste electrode

Acebutolol

Voltammetry

Oxidation

Urine analysis and blood plasma