Electro-Polymerized Poly(L-Arginine) Film as An Efficient Electrode Modifier for Highly Sensitive Determination of Methadone in Real Samples

Karim-Nezhad, Ghasem; Khorablou, Zeynab; Sadegh, Bayat; Mahmoudi, Tohid

Electro-Polymerized Poly(L-Arginine) Film as An Efficient Electrode Modifier for Highly Sensitive Determination of Methadone in Real Samples

Document Type : Original Article

Authors

Ghasem Karim-Nezhad

Zeynab Khorablou

Bayat Sadegh

Tohid Mahmoudi

Department of Chemistry, Payame Noor University, P.O. BOX 19395-3697 Tehran, Iran

Abstract

Developing simple yet efficient sensors for drug-abuse detection is highly required from both human health and medical aspects. In this work, a glassy carbon electrode modified by a thin layer of poly L-arginine (P-L-Arg/GCE) was fabricated by electropolymerization of L-arginine monomer using cyclic voltammetry (CV) and then it was used for determination of Methadone (MET). The surface characteristics of the P-L-Arg/GCE was examined by scanning electron microscopy and electrochemical impedance spectroscopy. To study the electrochemical responses of MET on P-L-Arg/GCE and bare GCE, cyclic and differential pulse voltammetric techniques were employed. For P-L-Arg/GCE, a considerable enhancement in electrooxidation signal of MET was observed compared to bare electrode. The influence of various determinant parameters on P-L-Arg/GCE performance was studied. At optimized conditions, using DPV technique, the electrooxidation peak current was linear to MET concentration in the ranges of 0.49-2.98 µM and 2.98-11.9 µM with a detection limit of 0.32 µM. The utility of P-L-Arg/GCE was successfully validated by analyzing MET in serum and urine samples, representing its potential application for real samples analysis.

Keywords

Methadone

Arginine

Electropolymerization

Modified glassy carbon electrode

Differential pulse voltammetry