Electrochemical Determination of Human Growth Hormone (HGH) Utilizing A Pyrrole (Py) Molecularly Imprinted Polymer (MIP) On Screen-Printed Carbon Electrode (SPCE)

Bohlooli, Shahriar; Kia, Solmaz; Bohlooli, Shahab; Sariri, Reyhaneh

doi:10.22034/abec.2023.705138

Electrochemical Determination of Human Growth Hormone (HGH) Utilizing A Pyrrole (Py) Molecularly Imprinted Polymer (MIP) On Screen-Printed Carbon Electrode (SPCE)

Document Type : Original Article

Authors

Shahriar Bohlooli ¹

Solmaz Kia ²

Shahab Bohlooli ³

Reyhaneh Sariri ¹

¹ Department of Biology, University of Guilan, Rasht, Iran

² Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran

³ Department of Pharmacology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran

10.22034/abec.2023.705138

Abstract

A new electrochemical biosensor based on Fe₃O₄ nanoparticles and molecularly imprinted polymer-modified screen-printed carbon electrode (SPCE) was built to record the HGH molecules. The HGH is an important metabolic hormone and is responsible for body growth, stimulating the growth and differentiation of muscle, bone, and cartilage. A stable SPCE/Fe3O4/Poly-pyrrole/MIP layer was formed by the electro-polymerization of pyrrole molecules as functional monomers and HGH as template molecules. The accuracy and sensitivity of the prepared SPCE were confirmed using the electrochemical impedance spectroscopy (EIS) technique. According to the results, the MIP showed a linear range of 1×
10^-6 to 1×10^-11 g/cm³for the HGH under study conditions with a limit of detection (LOD) of 2.927×10^-12 g/cm³. The calibration curve showed 99.9% correlation of the data. It can be concluded that the MIP made for HGH molecules has high sensitivity and stability, low cost, short response time, and excellent reproducibility.

Keywords

Screen-printed carbon electrode (SPCE)

Electrochemical impedance spectroscopy (EIS)

Human Growth Hormone (HGH)

Molecularly imprinted polymer

Electropolymerization

Subjects