Document Type : Original Article
Department of Biology, University of Guilan, Rasht, Iran
Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran
Department of Pharmacology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran
A new electrochemical biosensor based on Fe3O4 nanoparticles and molecularly imprinted polymer-modified screen-printed carbon electrode (SPCE) was built to record the HGH molecules. The HGH is an important metabolic hormone and is responsible for body growth, stimulating the growth and differentiation of muscle, bone, and cartilage. A stable SPCE/Fe3O4/Poly-pyrrole/MIP layer was formed by the electro-polymerization of pyrrole molecules as functional monomers and HGH as template molecules. The accuracy and sensitivity of the prepared SPCE were confirmed using the electrochemical impedance spectroscopy (EIS) technique. According to the results, the MIP showed a linear range of 1×
10-6 to 1×10-11 g/cm3 for the HGH under study conditions with a limit of detection (LOD) of 2.927×10-12 g/cm3. The calibration curve showed 99.9% correlation of the data. It can be concluded that the MIP made for HGH molecules has high sensitivity and stability, low cost, short response time, and excellent reproducibility.