Synthesis, Structural Determination, Electrochemical Investigation of Metal Complexes of Novel 4-[3-Hydroxypyridin-2-yl)diazenyl]-5-methyl-2-phenyl-2,4-dihydro-3H-pyrazol-3-one; Biological Evaluations

Anilkumara, H.A. .; Krishnamurthy, G .; Manjunatha, M. N.; Chavan, Prabhakar; Pari, Malathesh; Ranjitha, N; Khan, Fasiulla

doi:10.22034/abec.2024.714077

Synthesis, Structural Determination, Electrochemical Investigation of Metal Complexes of Novel 4-[3-Hydroxypyridin-2-yl)diazenyl]-5-methyl-2-phenyl-2,4-dihydro-3H-pyrazol-3-one; Biological Evaluations

Document Type : Original Article

Authors

H.A. . Anilkumara ¹

G . Krishnamurthy ¹

M. N. Manjunatha ²

Prabhakar Chavan ¹

Malathesh Pari ¹

N Ranjitha ¹

Fasiulla Khan ³

¹ Department of P.G. Studies and Research in Chemistry, Sahyadri Science College, Kuvempu University, Shivamogga -577 203, Karnataka, India

² Department of Chemistry, M S Ramaiah Institute of Technology, Bangalore-560054, Karnataka, India

³ Department of Chemistry, Manipal University, Manipal – 576104, Karnataka, India

10.22034/abec.2024.714077

Abstract

The pyrazole derivatives and its metal complexes are considered to be pharmacologically significant with a variety of pharmacological applications. The present investigation highlights the synthesis of pyrazole based novel ligand, 4-[(E)-(3-hydroxypyridin-2-yl)diazenyl]-5-methyl-2-phenyl-2,4-dihydro-3H-pyrazol-3-one (PDP) and its Co(II), Ni(II), Cu(II) complexes. The structure of the synthesized compounds has been evaluated using various physicochemical and spectroscopic techniques. Cyclic Voltammetry (CV) results are used to confirm the electrocatalytic ability of the modified electrode, [Co(PDP)₂]Cl₂.2H₂O/GCE for the detection of Folic acid (FA) at different concentrations. The CV results displayed good electro-catalytic activity with 10-120 µML^-1 with limits of detection (LOD) of 0.0666 µML^-1. This electrode has a sensitivity of 2.8784 µAµM^-1cm^-2 for FA. The bio-efficacy of the synthesized ligand and its complexes have been evaluated against the bacteria (S.aures and E.coli) and fungal (A.flavus and P.anomala) strains using standard method. Antidiabetic study on two different enzymes, α- amylase and α-Glucosidase indicating excellent inhibition by the metal complexes having the maximum IC₅₀ Value of 105.34 for α-amylase and 107.15 mg/mL for α- Glucosidase.

Keywords

Azo-dye

Metal complexes

Cyclic Voltammetry

Antidiabetics

Folic acid

Subjects