Document Type : Original Article
Authors
1
Pharmaceutical Chemistry Department, Egyptian Drug Authority, 51 Wezaret El-Zeraa St, Cairo, Egypt
2
Analytical Chemistry Department, Faculty of Pharmacy, Badr University in Cairo (BUC), Entertainment Area, Badr City, Cairo 11829, Egypt
3
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562, Cairo, Egypt
4
Pharmaceutical Chemistry Department, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt (FUE), 90th Street, Fifth Settlement, New Cairo 11835, Egypt
5
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif, Saudi Arabia
6
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Egypt
Abstract
COVID-19, a novel coronavirus, was identified as the primary source of pneumonia and severe acute respiratory syndrome. Roflumilast (ROF) is a widely used anti-inflammatory medication for controlling severe inflammatory pulmonary conditions. A novel sensor of sodium polystyrene sulfonate has been constructed to get an economic modifier for developing sensitive and swift voltammetric determination of ROF by square wave voltammetry in pharmaceutical dosage form and biological fluid. For obtaining best results, a universal buffer was used at pH=7. The calibration range was found to be 30×10-9 - 3×10-4 M and the correlation coefficient was computed to be 0.9995, under optimum conditions, while LOD and LOQ were found to be 9×10-9, 27×10-9 M respectively. Sensitive simultaneous determination of ROF and Salmeterol (SAL), the co-administered drug, was achieved efficiently by the sodium polystyrene sulfonate sensor. The modified electrode was proved to be the method of choice for analysis of ROF in the dosage forms and in human biological fluids due to high sensitivity, good accuracy, and specificity. Both the number of transported electrons and the potential for an electro-oxidation pathway were examined. Greenness assessment of the presented method was checked by applying NEMI, ESA, and GAPI tools and the method proved to be acceptably green.
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