Differential Pulse Voltammetric strategy for Simultaneous Determination of Aceclofenac and Ezogabine in Biological Fluids at Edge-plane Pyrolytic Graphite Electrode

Document Type : Original Article


1 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Zagazig University, Giza, Egypt

2 National Organization for drug control and research

3 Department of Analytical Chemistry, National Organization for Drug Control and Research (NODCAR), Giza, Egypt


Herein, an eco-friendly and rapid voltammetric method is suggested for simultaneous assay of Aceclofenac (ACL) and Ezogabine (EZO) in biological fluids. Aceclofenac (ACL) and Ezogabine (EZO) are co-administrative drugs which are prescribed for the treatment of epilepsy associated with chronic inflammatory. The voltammetric method is based on the electrochemical oxidation of ACL and EZO at an edge plane pyrolytic graphite electrode using differential pulse voltammetry (DPV). Moreover, the electrochemical oxidation at carbon paste electrode and carbon paste electrodes modified with nanoparticles have been remarked. A number of crucial parameters which affect the electrochemical process at the electrode surface have been optimized. The method is linear over the concentration ranges from 0.2 to 100.0 µM and from 2.0 to 70.0 µM for ACL and EZO, respectively. The average recovery was found to be 100±2% with satisfactory %RSD (