Analytical Determination of Paracetamol in Human Blood by Improving the Catalytic Effect of a Clay-Based Electrode

Hayat, EL Ouafy; Tarik, EL Ouafy; Mustapha, Oubenali; Mohamed, Mbarki; Malika, Echajia

Analytical Determination of Paracetamol in Human Blood by Improving the Catalytic Effect of a Clay-Based Electrode

Document Type : Original Article

Authors

EL Ouafy Hayat ¹

EL Ouafy Tarik ²

Oubenali Mustapha ¹

Mbarki Mohamed ¹

Echajia Malika ¹

¹ Laboratory of Organic and Analytical Chemistry, Sultan Moulay Slimane University, Faculty of Science and Technology, Beni Mellal, Morocco

² Laboratory of Organic and Analytical Chemistry, Sultan Moulay Slimane University, Polydisciplinary Faculty, Khouribga, Morocco

Abstract

In this work, we investigated the applicability of the clay-modified carbon paste electrode (CPE-clay) in the detection of paracetamol (PAR), using two electrochemical methods: differential pulse voltammetry (DPV) and cyclic voltammetry (CV) in the range between 20 and 400 mV in a Britton Robinson buffer solution (pH 7). The voltammetric technical behavior of PAR is studied where a sensitive anodic and cathodic peak has been appeared at about 0.4 V and 0.2 V (vs. Ag/AgCl/3 M KCl) successively. These peaks were recorded from the reversible redox of PAR at the CPE-clay surface. The proposed technical (DPV, CV) exhibits remarkably an electro-catalytic success for PAR redox. The current response of the catalytic peaks obtained by DPV depended linearly on the concentration of PAR in the range between 1.0 × 10-6 and 1.0 × 10-3 mol L-1 with a detection limit of 5.27 × 10-9 mol L-1. Subsequently, the relative standard deviation (RSD) at 1.0 × 10-4 mol L-1 PAR concentration was 3.8% for nine replicates. The proposed electrode has been successfully used for PAR detection in the human blood sample.

Keywords

Paracetamol

Clay

Human blood

Electroanalysis

Electrode