A New Efficient Agarose Gel based Electro-membrane To Extract, Preconcentrate, Clean-up and Quantify Nilotinib in Biological Samples

Document Type : Original Article


Department of Chemistry, Faculty of Science, University of Birjand, Birjand, South Khorasan, Iran


Abstract- For the first time, this study used agarose gel as a membrane in electro membrane extraction (AG-EME) without using any organic solvent to preconcentrate and clean up nilotinib in biological samples, following: its spectrofluorometric determination. Optimal conditions were: agarose concentration (w/v) 3.0%, acetic acid concentration in membrane (v/v) 0.2%, applied electric voltage 50V, pH of the donor phase (pHd) 4, pH of the acceptor phase (pHa) 3, and extraction time of 30 min. Limits of detection (LOD) and quantification (LOQ) were 15 and 50 ng mL˗1, and the analytical curve was linear at the range of 50–5000 ng mL˗1. The proposed validation method was compared with the other electro-membrane extraction techniques. Our proposed method is fast; it uses little sample and possesses a short extraction time. The developed procedure could be utilized successfully to determine the total amount of nilotinib, an anticancer drug, as a routine analysis in the biological samples.