Use of an Electrogenerated Chemiluminescence Sensor Modified with Sm2O3 Nanoparticles/Chitosan for the Analysis of Phenylalanine

Mohammad Beigi, Sepideh; Khurshid, Chrow Ahmed; Mesgari, Fazeleh; Poursaberi, Tahereh; Hosseini, Morteza

Use of an Electrogenerated Chemiluminescence Sensor Modified with Sm2O3 Nanoparticles/Chitosan for the Analysis of Phenylalanine

Document Type : Original Article

Authors

Sepideh Mohammad Beigi ¹

Chrow Ahmed Khurshid ²

Fazeleh Mesgari ¹

Tahereh Poursaberi ³

Morteza Hosseini ⁴

¹ Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran

² College of Pharmacy, University of Kirkuk, Iraq

³ Analytical Chemistry Research Center, Research Institute of Petroleum Industry, Tehran, Iran

⁴ Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran

Abstract

After modifying an electrogenerated chemiluminescence sensor with a Ru(bpy)32+/Sm2O3 Nanoparticles(NPs)/chitosan composite, the resulting electrode was used as a sensitive tool for the detection of phenylalanine. The electrochemiluminescence (ECL) signal resulting from the electrochemical interaction between tris(2,2-bipyridyl) ruthenium(II) (Ru(bpy)32+) and the analyte on the surface of the modified sensor. The presence of the Sm2O3NPs was found to enhance the ECL signal. Further the influences of Ru(bpy)32+ concentration, potential scan rate and solution pH on the intensity of the signal were monitored and optimized. The method showed a linear response in the range of 5.0×10-11 to 5.0×10-7 M (R2=0.9824) with a detection limit of 1.4×10-11 M. The modified ECL sensor was further used for the analysis of phenylalanine in human serum samples and an RSDs of 4.4% was observed, proving the high sensitivity, selectivity, and good stability of the sensor.

Keywords

Electrochemiluminescence

Phenylalanine

Samarium

Enhance

Subjects